Active Recombinant Human ANGPT2 Protein (Met1-Phe496), His-tagged

• Cat.No.: ANGPT2-415H
• Product Overview: Recombinant Human ANGPT2 Protein (NP_001138.1) (Met1-Phe496) was produced by CHO Stable Cells expression system. This protein was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: Met1-Phe496
• Form: Lyophilized from sterile 20 mM MOPS, 150 mM NaCl, 0.05 % CHAPS, pH 7.0.
• Bio-activity: Immobilized Human Ang2 His at 2 μg/ml (100 μl/well) can bind Human TIE2 Fch, the EC50 of Human TIE2 Fch is 80-480 ng/mL.
• Molecular Mass: The recombinant human ANGPT2 consists of 489 amino acids and predicts a molecular mass of 56.4 kDa. As a result of glycosylation, it migrates as an approximately 62.6 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ANGPT2 angiopoietin 2 [ Homo sapiens ]
• Official Symbol: ANGPT2
• Synonyms: ANGPT2; angiopoietin 2; angiopoietin-2; Ang2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a; ANG2; AGPT2
• Gene ID: 285
• mRNA Refseq: NM_001118887
• Protein Refseq: NP_001112359
• MIM: 601922
• UniProt ID: O15123