Recombinant Human ANXA1 293 Cell Lysate

Cat.No. : ANXA1-8839HCL
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  • Gene Information
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Description : Antigen standard for annexin A1 (ANXA1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ANXA1 annexin A1 [ Homo sapiens ]
Official Symbol : ANXA1
Synonyms : ANXA1; annexin A1; ANX1, LPC1; p35; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; annexin I (lipocortin I); phospholipase A2 inhibitory protein; ANX1; LPC1;
Gene ID : 301
mRNA Refseq : NM_000700
Protein Refseq : NP_000691
MIM : 151690
UniProt ID : P04083
Chromosome Location : 9q21.13
Pathway : Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Formyl peptide receptors bind formyl peptides and many other ligands, organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Peptide ligand-binding receptors, organism-specific biosystem;
Function : calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; phospholipase inhibitor activity; phospholipase inhibitor activity; phospholipid binding; protein binding; protein binding, bridging; protein homodimerization activity; receptor binding; single-stranded DNA binding; single-stranded RNA binding; structural molecule activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (13)

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How is the expression of ANXA1 protein regulated? 04/29/2023

The expression of ANXA1 protein can be regulated at the transcriptional and post-transcriptional levels. Transcriptional regulation involves the binding of transcription factors to specific regions of the ANXA1 gene promoter, which can either promote or inhibit its expression. Post-transcriptional regulation includes processes such as mRNA stability, alternative splicing, and microRNA-mediated regulation.

What are the clinical implications of ANXA1 protein? 04/02/2023

ANXA1 protein has been associated with various diseases and conditions. It has been found to play a role in cancer development and progression, inflammation-related disorders, cardiovascular diseases, and neurodegenerative diseases. It is also being investigated as a potential therapeutic target in these conditions.

Can ANXA1 protein be secreted from cells? 02/23/2023

Yes, ANXA1 can be secreted from cells. It is typically localized in the cytoplasm and can be translocated to the cell membrane in response to various stimuli. Upon activation, ANXA1 can undergo acetylation and be released from the cell, either as a free protein or associated with membrane-derived vesicles called exosomes.

Is ANXA1 protein used as a diagnostic marker? 02/22/2023

ANXA1 protein has been explored as a potential diagnostic marker for certain diseases. It has been studied as a biomarker for oral squamous cell carcinoma, breast cancer, and gastric cancer, among others. However, further research is needed to fully establish its diagnostic utility.

What are the cellular functions of ANXA1 protein? 03/13/2022

ANXA1 protein has diverse cellular functions. It is primarily known for its role in the resolution of inflammation. ANXA1 inhibits phospholipase A2 activity, leading to a decrease in prostaglandin and leukotriene synthesis, thereby suppressing inflammation. It also has anti-inflammatory effects by promoting neutrophil apoptosis and phagocytosis of apoptotic neutrophils by macrophages.

Are there any drugs targeting ANXA1 protein? 08/06/2020

Currently, there are no drugs specifically targeting ANXA1 protein approved for clinical use. However, ANXA1 has been considered as a potential target for the development of therapeutic agents. Various approaches have been explored, including the use of small molecule inhibitors, peptide analogs, and antibodies that can modulate ANXA1 function or its downstream signaling pathways. These strategies aim to exploit the role of ANXA1 in diseases such as inflammation, cancer, and cardiovascular disorders.

What are the interacting partners of ANXA1 protein? 03/19/2020

ANXA1 protein interacts with various proteins and molecules in the cell. Some of its interacting partners include actin, S100A11, S100A6, p11, p53, and integrins. These interactions are important for its cellular functions and signaling pathways.

How is ANXA1 protein involved in cancer progression and metastasis? 12/29/2018

ANXA1 protein has been implicated in cancer progression and metastasis through various mechanisms. It promotes tumor cell migration and invasion by modulating actin cytoskeletal dynamics and facilitating adhesion to extracellular matrix proteins. ANXA1 can also enhance tumor angiogenesis by promoting endothelial cell migration and tube formation.

Are there any genetic mutations or polymorphisms associated with ANXA1 protein? 05/21/2018

Yes, genetic mutations and polymorphisms in the ANXA1 gene have been reported in certain diseases, including certain types of cancer and autoimmune disorders. These genetic variations can affect the expression, structure, and function of the ANXA1 protein.

How is ANXA1 protein regulated? 03/28/2018

ANXA1 protein expression and activity can be regulated by various factors and signaling pathways. It can be upregulated by anti-inflammatory molecules, glucocorticoids, and certain growth factors. Conversely, it can be downregulated by pro-inflammatory cytokines and oxidative stress.

Are there any diseases associated with ANXA1 protein dysfunction? 03/22/2018

Dysregulation of ANXA1 protein has been implicated in several diseases. It has been associated with inflammation-related disorders such as asthma, inflammatory bowel disease, and rheumatoid arthritis. Alterations in ANXA1 expression and function have also been linked to cancer development, metastasis, and drug resistance. Additionally, ANXA1 dysfunction has been implicated in cardiovascular diseases, neurodegenerative disorders, and autoimmune diseases.

Can ANXA1 protein be a therapeutic target? 04/17/2017

ANXA1 protein is considered a potential therapeutic target due to its involvement in various disease processes. Targeting ANXA1 or its downstream signaling pathways could have therapeutic implications for inflammatory diseases, cancer treatment, and other disorders where its dysregulation is associated. However, more research is required to develop effective therapies targeting ANXA1.

Are there any therapeutic implications of ANXA1 protein? 02/05/2017

ANXA1 protein has potential therapeutic implications. It is being studied as a target for the development of anti-inflammatory drugs and cancer therapies. Additionally, ANXA1-based therapies, such as ANXA1 mimetics or ANXA1-derived peptides, are also being investigated for their therapeutic potential in various diseases and conditions. However, more research is needed in this area.

Customer Reviews (4)

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Reviews
10/30/2022

    Its purity and reliability ensure reliable and reproducible results in a variety of research applications.

    06/06/2020

      Its high quality greatly enhances the reliability and reproducibility of experimental data.

      10/26/2017

        ANXA1 protein is known for its versatility and functionality, enabling researchers to explore various biological processes and molecular interactions.

        04/23/2016

          Whether I need expertise in experimental optimization or troubleshooting, I can rely on the manufacturer's support to promptly address any concerns or queries.

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