Recombinant Human APAF1 Interacting Protein, His-tagged

• Cat.No.: APIP-6855H
• Product Overview: Recombinant human APIP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specification

• Description: APIP catalyzes the dehydration of methylthioribulose-1-phosphate into 2,3-diketo-5-methylthiopentyl-1-phosphate. It has an anti-apoptotic function and prevents muscle ischemic damage. This protein inhibits the cytochrome c-dependent and APAF1-mediated cell death.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT.
• Molecular Mass: 29.7 kDa (266aa) confirmed by MALDI-TOF
• Protein length: 1-242aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSGCDA REGDCCSRRC GAQDKEHPRY LIPELCKQFY HLGWVTGTGG GISLKHGDEI YIAPSGVQKE RIQPEDMFVC DINEKDISGP SPSKKLKKSQ CTPLFMNAYT MRGAGAVIHT HSKAAVMATL LFPGREFKIT HQEMIKGIKK CTSGGYYRYD DMLVVPIIEN TPEEKDLKDR MAHAMNEYPD SCAVLVRRHG VYVWGETWEK AKTMCECYDY LFDIAVSMKK VGLDPSQLPV GENGIV
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -76°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: APIP APAF1 interacting protein [ Homo sapiens ]
• Official Symbol: APIP
• Synonyms: APIP; APAF1 interacting protein; probable methylthioribulose-1-phosphate dehydratase; APIP2; CGI 29; Mmrp19; MTRu-1-P dehydratase; APAF1-interacting protein; CGI29; CGI-29; MMRP19; dJ179L10.2
• Gene ID: 51074
• mRNA Refseq: NM_015957
• Protein Refseq: NP_057041
• MIM: 612491
• UniProt ID: Q96GX9
• Chromosome Location: 11p13
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Metabolism of polyamines, organism-specific biosystem; Methionine salvage pathway, organism-specific biosystem
• Function: identical protein binding; ion binding; lyase activity; metal ion binding; methylthioribulose 1-phosphate dehydratase activity; protein binding