Recombinant Human APOE protein, His-tagged, Low Endotoxin

• Cat.No.: APOE-1377H
• Product Overview: Recombinant Human APOE protein(P02649-1)(Lys19-His317), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 19-317 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Molecular Mass: The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.
• Publications:
  • Imipramine and olanzapine block apoE4-catalyzed polymerization of Aβ and show evidence of improving Alzheimer’s disease cognition (2021)

Gene Information

• Gene Name: APOE apolipoprotein E [ Homo sapiens ]
• Official Symbol: APOE
• Synonyms: APOE; apolipoprotein E; AD2, Alzheimer disease 2 (APOE*E4 associated, late onset); apo-E; apolipoprotein E3; AD2; LPG; LDLCQ5; MGC1571
• Gene ID: 348
• mRNA Refseq: NM_000041
• Protein Refseq: NP_000032
• UniProt ID: P02649