Active Recombinant Human APOH Protein, His-tagged

• Cat.No.: APOH-308H
• Product Overview: Recombinant human APOH (NP_000033.2) (Met 1-Cys 345) was expressed, with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Gly 20
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.2. Immobilized human APOH-his at 2 μg/mL (100 μl/well) can bind biotinylated human LDLR-his, The EC50 of biotinylated human LDLR-his is 0.35 μg/mL.3.Immobilized human APOH-his at 2 μg/mL (100 μl/we
• Molecular Mass: The recombinant human APOH consists of 337 amino acids and predictes a molecular mass of 37.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhAPOH is approximately 53 kDa due to different glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >96 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: APOH apolipoprotein H (beta-2-glycoprotein I) [ Homo sapiens ]
• Official Symbol: APOH
• Synonyms: APOH; apolipoprotein H (beta-2-glycoprotein I); B2G1; beta-2-glycoprotein 1; beta 2 glycoprotein I; BG; B2GPI; apo-H; beta(2)GPI; APC inhibitor; anticardiolipin cofactor; activated protein C-binding protein; B2GP1
• Gene ID: 350
• mRNA Refseq: NM_000042
• Protein Refseq: NP_000033
• MIM: 138700
• UniProt ID: P02749
• Chromosome Location: 17q23-qter