Active Recombinant Human APOH protein, His-tagged, Low Endotoxin

• Cat.No.: APOH-308H
• Product Overview: Recombinant Human APOH protein(P02749)(Gly20-Cys345), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 20-345 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human APOH, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 37.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: APOH apolipoprotein H (beta-2-glycoprotein I) [ Homo sapiens ]
• Official Symbol: APOH
• Synonyms: APOH; apolipoprotein H (beta-2-glycoprotein I); B2G1; beta-2-glycoprotein 1; beta 2 glycoprotein I; BG; B2GPI; apo-H; beta(2)GPI; APC inhibitor; anticardiolipin cofactor; activated protein C-binding protein; B2GP1
• Gene ID: 350
• mRNA Refseq: NM_000042
• Protein Refseq: NP_000033
• MIM: 138700
• UniProt ID: P02749