| Description : |
Involved in pre-mRNA splicing as component of the spliceosome. Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP (small nucleolar ribonucleoprotein) biogenesis. Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly. May act by helping the folding of the snoRNA sequence. Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing. Has ATP-dependent RNA helicase activity and can unwind double-stranded RNA molecules with a 3' overhang (in vitro). |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
171.3 kDa |
| AA Sequence : |
MAAPAQPKKIVAPTVSQINAEFVTQ LACKYWAPHIKKKSPFDIKVIEDIY EKEIVKSRFAIRKIMLLEFSQYLEN YLWMNYSPEVSSKAYLMSICCMVNE KFRENVPAWEIFKKKPDHFPFFFKH ILKAALAETDGEFSLHEQTVLLLFL DHCFNSLEVDLIRSQVQQLISLPMW MGLQLARLELELKKTPKLRKFWNLI KKNDEKMDPEAREQAYQERRFLSQL IQKFISVLKSVPLSEPVTMDKVHYC ERFIELMIDLEALLPTRRWFNTILD DSHLLVHCYLSNLVRREEDGHLFSQ LLDMLKFYTGFEINDQTGNALTENE MTTIHYDRITSLQRAAFAHFPELYD FALSNVAEVDTRESLVKFFGPLSSN TLHQVASYLCLLPTLPKNEDTTFDK EFLLELLVSRHERRISQIQQLNQMP LYPTEKIIWDENIVPTEYYSGEGCL ALPKLNLQFLTLHDYLLRNFNLFRL ESTYEIRQDIEDSVSRMKPWQSEYG GVVFGGWARMAQPIVAFTVVEVAKP NIGENWPTRVRADVTINLNVRDHIK DEWEGLRKHDVCFLITVRPTKPYGT KFDRRRPFIEQVGLVYVRGCEIQGM LDDKGRVIEDGPEPRPNLRGESRTF RVFLDPNQYQQDMTNTIQNGAEDVY ETFNIIMRRKPKENNFKAVLETIRN LMNTDCVVPDWLHDIILGYGDPSSA HYSKMPNQIATLDFNDTFLSIEHLK ASFPGHNVKVTVEDPALQIPPFRIT FPVRSGKGKKRKDADVEDEDTEEAK TLIVEPHVIPNRGPYPYNQPKRNTI QFTHTQIEAIRAGMQPGLTMVVGPP GTGKTDVAVQIISNIYHNFPEQRTL IVTHSNQALNQLFEKIMALDIDERH LLRLGHGEEELETEKDFSRYGRVNY VLARRIELLEEVKRLQKSLGVPGDA SYTCETAGYFFLYQVMSRWEEYISK VKNKGSTLPDVTEVSTFFPFHEYFA NAPQPIFKGRSYEEDMEIAEGCFRH IKKIFTQLEEFRASELLRSGLDRSK YLLVKEAKIIAMTCTHAALKRHDLV KLGFKYDNILMEEAAQILEIETFIP LLLQNPQDGFSRLKRWIMIGDHHQL PPVIKNMAFQKYSNMEQSLFTRFVR VGVPTVDLDAQGRARASLCNLYNWR YKNLGNLPHVQLLPEFSTANAGLLY DFQLINVEDFQGVGESEPNPYFYQN LGEAEYVVALFMYMCLLGYPADKIS ILTTYNGQKHLIRDIINRRCGNNPL IGRPNKVTTVDRFQGQQNDYILLSL VRTRAVGHLRDVRRLVVAMSRARLG LYIFARVSLFQNCFELTPAFSQLTA RPLHLHIIPTEPFPTTRKNGERPSH EVQIIKNMPQMANFVYNMYMHLIQT THHYHQTLLQLPPAMVEEGEEVQNQ ETELETEEEAMTVQADIIPSPTDTS CRQETPAFQTDTTPSETGATSTPEA IPALSETTPTVVGAVSAPAEANTPQ DATSAPEETKTRTRPLEQKLISEED LAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
