Recombinant Human ARHGAP19 Protein (1-494 aa), His-SUMO-tagged

• Cat.No.: ARHGAP19-1015H
• Product Overview: Recombinant Human ARHGAP19 Protein (1-494 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Cell Cycle. Protein Description: Full Length.

Specification

• Description: Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-rodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the rodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin rodeling complex (npBAF complex) and the neuron-specific chromatin rodeling complex (nBAF complex). During neural development a switch from a st/progenitor to a post-mitotic chromatin rodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural st/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural st cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth . Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 60.1 kDa
• Protein length: 1-494 aa
• AA Sequence: MMMMALSKTFGQKPVKFQLEDDGEF YMIGSEVGNYLRMFRGSLYKRYPSL WRRLATVEERKKIVASSHGKKTKPN TKDHGYTTLATSVTLLKASEVEEIL DGNDEKYKAVSISTEPPTYLREQKA KRNSQWVPTLPNSSHHLDAVPCSTT INRNRMGRDKKRTFPLCFDDHDPAV IHENASQPEVLVPIRLDMEIDGQKL RDAFTWNMNEKLMTPEMFSEILCDD LDLNPLTFVPAIASAIRQQIESYPT DSILEDQSDQRVIIKLNIHVGNISL VDQFEWDMSEKENSPEKFALKLCSE LGLGGEFVTTIAYSIRGQLSWHQKT YAFSENPLPTVEIAIRNTGDADQWC PLLETLTDAEMEKKIRDQDRNTRRM RRLANTAPAW
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: ARHGAP19 Rho GTPase activating protein 19 [ Homo sapiens ]
• Official Symbol: ARHGAP19
• Synonyms: ARHGAP19; FLJ00194; MGC14258; MGC138804; MGC138805; DKFZp313K217
• Gene ID: 84986
• mRNA Refseq: NM_001204300
• Protein Refseq: NP_001191229
• MIM: 611587
• UniProt ID: Q14CB8