| Description : |
Contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
124.6 kDa |
| AA Sequence : |
MFSRKKRELMKTPSISKKNRAGSPS PQPSGELPRKDGADAVFPGPSLEPP AGSSGVKATGTLKRPTSLSRHASAA GFPLSGAASWTLGRSHRSPLTAASP GELPTEGAGPDVVEDISHLLADVAR FAEGLEKLKECVLHDDLLEARRPRA HECLGEALRVMHQIISKYPLLNTVE TLTAAGTLIAKVKAFHYESNNDLEK QEFEKALETIAVAFSSTVSEFLMGE VDSSTLLAVPPGDSSQSMESLYGPG SEGTPPSLDDCDAGCLPAEEVDVLL QRCEGGVDAALLYAKNMAKYMKDLI SYLEKRTTLEMEFAKGLQKIAHNCR QSVMQEPHMPLLSIYSLALEQDLEF GHSMVQAVGTLQTQTFMQPLTLRRL EHEKRRKEIKEAWHRAQRKLQEAES NLRKAKQGYVQRCEDHDKARFLVAK AEEEQAGSAPGAGGTATKTLDKRRR LEEEAKNKAEEAMATYRTCVADAKT QKQELEDTKVTALRQIQEVIRQSDQ TIKSATISYYQMMHMQTAPLPVHFQ MLCESSKLYDPGQQYASHVRQLQRD QEPDVHYDFEPHVSANAWSPVMRAR KSSFNVSDVARPEAAGSPPEEGGCT EGTPAKDHRAGRGHQVHKSWPLSIS DSDSGLDPGPGAGDFKKFERTSSSG TMSSTEELVDPDGGAGASAFEQADL NGMTPELPVAVPSGPFRHEGLSKAA RTHRLRKLRTPAKCRECNSYVYFQG AECEECCLACHKKCLETLAIQCGHK KLQGRLQLFGQDFSHAARSAPDGVP FIVKKCVCEIERRALRTKGIYRVNG VKTRVEKLCQAFENGKELVELSQAS PHDISNVLKLYLRQLPEPLISFRLY HELVGLAKDSLKAEAEAKAASRGRQ DGSESEAVAVALAGRLRELLRDLPP ENRASLQYLLRHLRRIVEVEQDNKM TPGNLGIVFGPTLLRPRPTEATVSL SSLVDYPHQARVIETLIVHYGLVFE EEPEETPGGQDESSNQRAEVVVQVP YLEAGEAVVYPLQEAAADGCRESRV VSNDSDSDLEEASELLSSSEASALG HLSFLEQQQSEASLEVASGSHSGSE EQLEATAREDGDGDEDGPAQQLSGF NTNQSNNVLQAPLPPMRLRGGRMTL GSCRERQPEFVSGPTRTRPLEQKLI SEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
