Recombinant Human ARHGEF18 Protein (159-551 aa), GST-tagged

• Cat.No.: ARHGEF18-1214H
• Product Overview: Recombinant Human ARHGEF18 Protein (159-551 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Research Area: Signal Transduction. Protein Description: Partial.

Specification

• Description: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 71.5 kDa
• Protein length: 159-551 aa
• AA Sequence: MTVSQKGGPQPTPSPAGPGTQLGPI TGEMDEADSAFLKFKQTADDSLSLT SPNTESIFVEDPYTASLRSEIESDG HEFEAESWSLAVDAAYAKKQKREVV KRQDVLYELMQTEVHHVRTLKIMLK VYSRALQEELQFSSKAIGRLFPCAD DLLETHSHFLARLKERRQESLEEGS DRNYVIQKIGDLLVQQFSGENGERM KEKYGVFCSGHNEAVSHYKLLLQQN KKFQNLIKKIGNFSIVRRLGVQECI LLVTQRITKYPVLVERIIQNTEAGT EDYEDLTQALNLIKDIISQVDAKVS ECEKGQRLREIAGKMDLKSSSKLKN GLTFRKEDMLQRQLHLEGMLCWKTT SGRLKDILAILLTDVLLLLQEKDQK YVFASVDSKPPVISLQKL
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: ARHGEF18 Rho/Rac guanine nucleotide exchange factor (GEF) 18 [ Homo sapiens ]
• Official Symbol: ARHGEF18
• Synonyms: ARHGEF18; KIAA0521; MGC15913; P114 RhoGEF; SA-RhoGEF; p114RhoGEF; P114-RHO-GEF; P114-RhoGEF
• Gene ID: 23370
• mRNA Refseq: NM_001130955
• Protein Refseq: NP_001124427
• UniProt ID: Q6ZSZ5