Recombinant Human ATF4, His-tagged

• Cat.No.: ATF4-27047TH
• Product Overview: Recombinant full length Human ATF4 with an N terminal His-Calmodulin tag; 510 amino acids with tag, Predicted MWt 56.6 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 351 amino acids
• Description: This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.
• Conjugation: HIS
• Molecular Weight: 56.600kDa inclusive of tags
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MHHHHHHMADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAKGSHMTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDLKEFDLDALLGIDDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP
• Sequence Similarities: Belongs to the bZIP family.Contains 1 bZIP domain.

Gene Information

• Gene Name: ATF4 activating transcription factor 4 (tax-responsive enhancer element B67) [ Homo sapiens ]
• Official Symbol: ATF4
• Synonyms: ATF4; activating transcription factor 4 (tax-responsive enhancer element B67); TXREB; cyclic AMP-dependent transcription factor ATF-4; CREB 2; TAXREB67
• Gene ID: 468
• mRNA Refseq: NM_001675
• Protein Refseq: NP_001666
• MIM: 604064
• Uniprot ID: P18848
• Chromosome Location: 22q13.1
• Pathway: Cholinergic synapse, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem; GnRH signaling pathway, organism-specific biosystem
• Function: DNA binding; DNA binding; protein binding; protein dimerization activity; sequence-specific DNA binding