Recombinant Human ATG13 protein, His-tagged

• Cat.No.: ATG13-12H
• Product Overview: Recombinant Mouse SEZ6 protein(Q7TSK2)(Leu20-His922) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Leu20-His922
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse SEZ6, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 98.8 kDa. Due to glycosylation, the protein migrates to 145-150 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt; -80°C for 3-6 months after reconstitution; 2-8°C for 2-7 days after reconstitution; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.