Recombinant Human ATP1A2 293 Cell Lysate

• Cat.No.: ATP1A2-8612HCL

Specification

• Description: Antigen standard for ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide (ATP1A2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: ATP1A2 ATPase, Na+/K+ transporting, alpha 2 polypeptide [ Homo sapiens ]
• Official Symbol: ATP1A2
• Synonyms: ATP1A2; ATPase, Na+/K+ transporting, alpha 2 polypeptide; ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide , MHP2, migraine, hemiplegic 2; sodium/potassium-transporting ATPase subunit alpha-2; FHM2; sodium-potassium ATPase; sodium pump subunit alpha-2; Na(+)/K(+) ATPase alpha-2 subunit; Na+/K+ ATPase, alpha-B polypeptide; Na+/K+ ATPase, alpha-A(+) catalytic polypeptide; sodium/potassium-transporting ATPase alpha-2 chain; MHP2; MGC59864
• Gene ID: 477
• mRNA Refseq: NM_000702
• Protein Refseq: NP_000693
• MIM: 182340
• UniProt ID: P50993
• Chromosome Location: 1q21-q23
• Pathway: Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Carbohydrate digestion and absorption, organism-specific biosystem; Carbohydrate digestion and absorption, conserved biosystem; Cardiac muscle contraction, organism-specific biosystem
• Function: ATP binding; ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism; cation-transporting ATPase activity; hydrolase activity; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances; metal ion binding; monovalent inorganic cation transmembrane transporter activity; nucleotide binding; sodium:potassium-exchanging ATPase activity; sodium:potassium-exchanging ATPase activity