Recombinant Human ATP5C1Protein, His-tagged

• Cat.No.: ATP5C1-132H
• Product Overview: Recombinant Human ATP5C1, transcript variant 1, fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.
• Molecular Mass: 30.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: ATP5C1 ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 [ Homo sapiens ]
• Official Symbol: ATP5C1
• Synonyms: ATP5C1; ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1; ATP5C, ATP5CL1; ATP synthase subunit gamma, mitochondrial; F-ATPase gamma subunit; ATP synthase gamma chain, mitochondrial; mitochondrial ATP synthase, gamma subunit 1; ATP5C; ATP5CL1
• Gene ID: 509
• mRNA Refseq: NM_001001973
• Protein Refseq: NP_001001973
• MIM: 108729
• UniProt ID: P36542