Recombinant Human ATP5J protein, His & T7-tagged

• Cat.No.: ATP5J-1531H
• Product Overview: Recombinant Human ATP5J aa. (Met1~Ala108 (Accession # P18859)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.3kDa
• Protein length: Met1~Ala108 (Accession # P18859)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 9.5

Gene Information

• Gene Name: ATP5J ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 [ Homo sapiens (human) ]
• Official Symbol: ATP5J
• Synonyms: ATP5J; ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6; F6; CF6; ATP5; ATPM; ATP5A; ATP synthase-coupling factor 6, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6; ATPase subunit F6; coupling factor 6; mitochondrial ATP synthase, coupling factor 6; mitochondrial ATP synthase, subunit F6; mitochondrial ATPase coupling factor 6; proliferation-inducing protein 36
• Gene ID: 522
• mRNA Refseq: NM_001003696.1
• Protein Refseq: NP_001003696.1
• UniProt ID: P18859