Recombinant Human ATP6V0A1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ATP6V0A1-2980H
• Product Overview: ATP6V0A1 MS Standard C13 and N15-labeled recombinant protein (NP_005168) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: 95.6 kDa
• AA Sequence: MGELFRSEEMTLAQLFLQSEAAYCCVSELGELGKVQFRDLNPDVNVFQRKFVNEVRRCEEMDRKLRFVEKEIRKANIPIMDTGENPEVPFPRDMIDLEANFEKIENELKEINTNQEALKRNFLELTELKFILRKTQQFFDEMADPDLLEESSSLLEPSEMGRGTPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQAEIENPLEDPVTGDYVHKSVFIIFFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMASGVNTRIDDLQMVLNQTEDHRQRVLQAAAKNIRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPVTDLDSIQFALRRGTEHSGSTVPSILNRMQTNQTPPTYNKTNKFTYGFQNIVDAYGIGTYREINPAPYTIITFPFLFAVMFGDFGHGILMTLFAVWMVLRESRILSQKNENEMFSTVFSGRYIILLMGVFSMYTGLIYNDCFSKSLNIFGSSWSVRPMFTYNWTEETLRGNPVLQLNPALPGVFGGPYPFGIDPIWNIATNKLTFLNSFKMKMSVILGIIHMLFGVSLSLFNHIYFKKPLNIYFGFIPEIIFMTSLFGYLVILIFYKWTAYDAHTSENAPSLLIHFINMFLFSYPESGYSMLYSGQKGIQCFLVVVALLCVPWMLLFKPLVLRRQYLRRKHLGTLNFGGIRVGNGPTEEDAEIIQHDQLSTHSEDADEFDFGDTMVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVIHIGLSVKSLAGGLVLFFFFTAFATLTVAILLIMEGLSAFLHALRLHWVEFQNKFYSGTGFKFLPFSFEHIREGKFEETRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ATP6V0A1 ATPase H+ transporting V0 subunit a1 [ Homo sapiens (human) ]
• Official Symbol: ATP6V0A1
• Synonyms: ATP6V0A1; ATPase, H+ transporting, lysosomal V0 subunit a1; ATP6N1, ATP6N1A, ATPase, H+ transporting, lysosomal (vacuolar proton pump) non catalytic accessory protein 1A (110/116kD), ATPase, H+ transporting, lysosomal V0 subunit a isoform 1, ATPase, H+ transporting, lysosomal V0 subunit A1, VPP1; V-type proton ATPase 116 kDa subunit a isoform 1; a1; Stv1; Vph1; V-ATPase 116 kDa; vacuolar proton pump subunit 1; vacuolar proton pump, subunit 1; V-type proton ATPase 116 kDa subunit a; vacuolar-type H(+)-ATPase 115 kDa subunit; vacuolar adenosine triphosphatase subunit Ac116; vacuolar proton translocating ATPase 116 kDa subunit A; H(+)-transporting two-sector ATPase, 116 kDa accessory protein A1; clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit; ATPase, H+ transporting, lysosomal non-catalytic accessory protein 1 (110/116kD); ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 1A (110/116kD); VPP1; ATP6N1; ATP6N1A; DKFZp781J1951
• Gene ID: 535
• mRNA Refseq: NM_005177
• Protein Refseq: NP_005168
• MIM: 192130
• UniProt ID: Q93050