Recombinant Human ATP6V1F, GST-tagged

• Cat.No.: ATP6V1F-226H
• Product Overview: A DNA sequence encoding the human ATP6V1F (NP_004222.2) (Met1-Arg119) was expressed with the GST tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: Met1-Arg119
• Form: Lyophilized from sterile PBS, pH7.4.
• Molecular Mass: The recombinant human ATP6V1F/GST chimera consists of 350 amino acids and has a predicted molecular mass of 40.2 KDa. It migrates as an approximately 40 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: ATP6V1F ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F [ Homo sapiens ]
• Official Symbol: ATP6V1F
• Synonyms: ATP6V1F; ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F; V-type proton ATPase subunit F; ATP6S14; VATF; Vma7; V-ATPase F subunit; V-ATPase subunit F; ATPase, vacuolar, 14 kD; V-ATPase 14 kDa subunit; vacuolar proton pump F subunit; vacuolar proton pump subunit F; vacuolar ATP synthase subunit F; adenosinetriphosphatase 14k chain; H(+)-transporting two-sector ATPase, 14kD subunit; MGC117321; MGC126037; MGC126038
• Gene ID: 9296
• mRNA Refseq: NM_001198909
• Protein Refseq: NP_001185838
• MIM: 607160
• UniProt ID: Q16864
• Chromosome Location: 7q32.1
• Pathway: Collecting duct acid secretion, organism-specific biosystem; Collecting duct acid secretion, conserved biosystem; Epithelial cell signaling in Helicobacter pylori infection, organism-specific biosystem; Epithelial cell signaling in Helicobacter pylori infection, conserved biosystem; Insulin receptor recycling, organism-specific biosystem; Iron uptake and transport, organism-specific biosystem; Metabolic pathways, organism-specific biosystem
• Function: ATPase activity, uncoupled; hydrogen ion transmembrane transporter activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; protein binding; proton-transporting ATPase activity, rotational mechanism