Recombinant Human AXL protein, His-tagged

• Cat.No.: AXL-9382H
• Product Overview: Recombinant Human AXL protein(NP_001265528.1)(Gly29~Met269) is expressed from E. coli with a N-terminal His Tag.

Specification

• Description: The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gly29~Met269
• AA Sequence: GTQAEESPFVGNPGNITGARGLTGT LRCQLQVQGEPPEVHWLRDGQILEL ADSTQTQVPLGEDEQDDWIVVSQLR ITSLQLSDTGQYQCLVFLGHQTFVS QPGYVGLEGLPYFLEEPEDRTVAAN TPFNLSCQAQGPPEPVDLLWLQDAV PLATAPGHGPQRSLHVPGLNKTSSF SCEAHNAKGVTTSRTATITVLPQQP RNLHLVSRQPTELEVAWTPGLSGIY PLTHCTLQAVLSDDGM
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  (May be suitable for use in other assays to be determined by the end user.)
• Notes: The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles.
  Store at 2-8°C for one month.
  Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: AXL AXL receptor tyrosine kinase[Homo sapiens (human)]
• Official Symbol: AXL
• Synonyms: UFO; JTK11; Tyrosine-protein kinase receptor UFO
• Gene ID: 558
• mRNA Refseq: NM_001278599.2
• Protein Refseq: NP_001265528.1
• MIM: 109135
• UniProt ID: P30530