Recombinant Human BAG6 Protein (1-321 aa), His-Myc-tagged

• Cat.No.: BAG6-2593H
• Product Overview: Recombinant Human BAG6 Protein (1-321 aa) is produced by E. coli expression system. This protein is fused with a 10xHis tag at the N-terminal and a Myc tag at the C-terminal. Research Area: Cancer. Protein Description: Partial.

Specification

• Description: ATP-independent molecular chaperone preventing the aggregation of misfolded and hydrophobic patches-containing proteins. Functions as part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, which maintains these client proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation. The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum. Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated by RNF126, an E3 ubiquitin-protein ligase associated with BAG6 and are sorted to the proteasome. SGTA which prevents the recruitment of RNF126 to BAG6 may negatively regulate the ubiquitination and the proteasomal degradation of client proteins, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum. The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome. BAG6 is also required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, it may participate to the production of antigenic peptides and play a role in antigen presentation in immune response. BAG6 is also involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. BAG6 may ensure the proper degradation of these proteins and thereby protects the endoplasmic reticulum from protein overload upon stress. By inhibiting the polyubiquitination and subsequent proteasomal degradation of HSPA2 it may also play a role in the assembly of the synaptonemal complex during spermatogenesis. Also positively regulates apoptosis by interacting with and stabilizing the proapoptotic factor AIFM1.
• Source: E. coli
• Species: Human
• Tag: His-Myc
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 41.7 kDa
• Protein length: 1-321 aa
• AA Sequence: MEPNDSTSTAVEEPDSLEVLVKTLD SQTRTFIVGAQMNVKEFKEHIAASV SIPSEKQRLIYQGRVLQDDKKLQEY NVGGKVIHLVERAPPQTHLPSGASS GTGSASATHGGGSPPGTRGPGASVH DRNANSYVMVGTFNLPSDGSAVDVH INMEQAPIQSEPRVRLVMAQHMIRD IQTLLSRMECRGGPQPQHSQPPPQP PAVTPEPVALSSQTSEPVESEAPPR EPMEAEEVEERAPAQNPELTPGPAP AGPTPAPETNAPNHPSPAEYVEVLQ ELQRLESRLQPFLQRYYEVLGAAAT TDYNNNHEGREEDQRLINLVG
• Purity: > 85% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: BAG6 BCL2-associated athanogene 6 [ Homo sapiens ]
• Official Symbol: BAG6
• Synonyms: BAG6; BCL2-associated athanogene 6; D6S52E; G3; scythe; protein G3; protein Scythe; BAT3; BAG-6
• Gene ID: 7917
• mRNA Refseq: NM_001098534
• Protein Refseq: NP_001092004
• MIM: 142590
• UniProt ID: P46379