Recombinant Human BBC3 293 Cell Lysate

• Cat.No.: BBC3-8505HCL

Specification

• Description: Antigen standard for BCL2 binding component 3 (BBC3), transcript variant 4 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: BBC3 BCL2 binding component 3 [ Homo sapiens ]
• Official Symbol: BBC3
• Synonyms: BBC3; BCL2 binding component 3; bcl-2-binding component 3; JFY1; PUMA; p53 up-regulated modulator of apoptosis; JFY-1; FLJ42994
• Gene ID: 27113
• mRNA Refseq: NM_001127240
• Protein Refseq: NP_001120712
• MIM: 605854
• UniProt ID: Q96PG8
• Chromosome Location: 19q13.3-q13.4
• Pathway: Activation of BH3-only proteins, organism-specific biosystem; Activation of PUMA and translocation to mitochondria, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; Huntingtons disease, organism-specific biosystem
• Function: protein binding