Recombinant Human BCAT1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: BCAT1-1842H
• Product Overview: BCAT1 MS Standard C13 and N15-labeled recombinant protein (NP_005495) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 42.8 kDa
• AA Sequence: MKDCSNGCSAECTGEGGSKEVVGTF KAKDLIVTPATILKEKPDPNNLVFG TVFTDHMLTVEWSSEFGWEKPHIKP LQNLSLHPGSSALHYAVELFEGLKA FRGVDNKIRLFQPNLNMDRMYRSAV RATLPVFDKEELLECIQQLVKLDQE WVPYSTSASLYIRPTFIGTEPSLGV KKPTKALLFVLLSPVGPYFSSGTFN PVSLWANPKYVRAWKGGTGDCKMGG NYGSSLFAQCEAVDNGCQQVLWLYG EDHQITEVGTMNLFLYWINEDGEEE LATPPLDGIILPGVTRRCILDLAHQ WGEFKVSERYLTMDDLTTALEGNRV REMFGSGTACVVCPVSDILYKGETI HIPTMENGPKLASRILSKLTDIQYG REESDWTIVLSTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: BCAT1 branched chain amino acid transaminase 1 [ Homo sapiens (human) ]
• Official Symbol: BCAT1
• Synonyms: BCAT1; branched chain amino-acid transaminase 1, cytosolic; BCT1, branched chain aminotransferase 1, cytosolic; branched-chain-amino-acid aminotransferase, cytosolic; placental protein 18; branched chain aminotransferase 1, cytosolic; BCT1; PP18; BCATC; ECA39; MECA39; PNAS121; DKFZp686E12175
• Gene ID: 586
• mRNA Refseq: NM_005504
• Protein Refseq: NP_005495
• MIM: 113520
• UniProt ID: P54687