Recombinant Human C-type Lectin Domain Family 11, Member A

• Cat.No.: CLEC11A-1409H
• Product Overview: Recombinant human C-type Lectin Domain Family 11, Member A was expressed inE.Coli. MW=33.9 kDa.

Specification

• Cat. No.: CLEC11A-1409H
• Description: Human stem cell growth factor α (SCGF-α) is a 33.9 kDa polypeptide containing 305 amino acid residues. SCGF-α and SCGF-β are hematopoietic growth factors that exert their activity at the early stages of hematopoiesis.
• Source: E.Coli.
• Specific Activity: ≥1×10μU/mg.
• Product Format: Sterile filtered, lyophilized.
• Stabilizer: None.
• Purity: >98% (SDS-PAGE, HPLC).
• Endotoxin Level: <0.1 ng/μg (1 EU/μg).
• Buffer: PBS (pH 7.3).
• Biological Activity: The ED₅₀of ≤10 ng/mL was determined by dose-dependent inhibition of the proliferation of human TF-1 cells, previously grown in media containing GM-CSF.
• Reconstitution: We recommend a quick spin followed by reconstitution in deionized sterile-filtered water to a concentration of 0.1–1.0 mg/mL. This protein is slow to dissolve. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or –20 °C for future use
• Storage: The lyophilized protein, though stable at room temperature, is best stored at –20 °C. Reconstituted Human SCGF-α should be stored in working aliquots at –20 °C. Avoid repeated freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: CLEC11A C-type lectin domain family 11, member A [ Homo sapiens ]
• Synonyms: CLEC11A; C-type lectin domain family 11, member A; P47; SCGF; LSLCL; CLECSF3; stem cell growth factor; stem cell growth factor; lymphocyte secreted C-type lectin; lymphocyte secreted long form of C-type lectin; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 3
• Gene ID: 6320
• mRNA Refseq: NM_002975
• Protein Refseq: NP_002966
• MIM: 604713
• UniProt ID: Q9Y240
• Chromosome Location: 19q13
• Function: binding; growth factor activity; sugar binding

Citation

MICL controls inflammation in rheumatoid arthritis

Journal: Annals of the Rheumatic Diseases    PubMed ID: 26275430    Data: 2016/7/1

Authors: Pierre Redelinghuys, Lauren Whitehead, Gordon D Brown

Article Snippet:Specificity of this assay could be demonstrated by inhibition of serum antibody binding with the addition of soluble Fc-MICL (see online supplementary figure S2B).Specificity of this assay could be demonstrated by inhibition of serum antibody binding with the addition of soluble Fc-MICL (see online supplementary figure S2B).. For dot blots, serum samples were used to probe recombinant histidine-tagged hMICL or histidine-tagged human C-type lectin superfamily member 8 (ClecSF8; Creative BioMart), immobilised on nitrocellulose membranes and then detected with peroxidase-conjugated goat anti-human IgG F(ab′) 2 fragment (Jackson ImmunoResearch) or mouse anti-His monoclonal antibody (Qiagen).. Serum samples from patients with RA (see online supplementary table S3) were obtained with consent from the Institute of Infection, Immunity and Inflammation Research Tissue Bank, Gartnavel General Hospital, Glasgow, UK and with ethical approval from the West of Scotland research ethics service.Serum samples from patients with RA (see online supplementary table S3) were obtained with consent from the Institute of Infection, Immunity and Inflammation Research Tissue Bank, Gartnavel General Hospital, Glasgow, UK and with ethical approval from the West of Scotland research..