Active Recombinant Human C1QC Protein, His-GST-tagged

• Cat.No.: C1QC-335H
• Product Overview: Active Recombinant Human C1QC Protein(117-245 aa), fused with N-terminal His and GST tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: 117-245 aa
• Form: PBS, pH7.4, containing 0.01%Sarcosyl, 5% Trehalose.
• Bio-activity: Complement component 1Q subcomponent C (C1qC) is an important part of the C1 complex in the classical activation pathway of the complement system. C1qC contains multiple domains that recognize and bind to pathogen surfaces or antibody-antigen complexes.C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. Thus a functional ELISA assay was conducted to detect the interaction of recombinant human C1qC and recombinant mouse C1r. Briefly, C1qC was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to C1r-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-C1qC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human C1qC and recombinant mouse C1r was shown, the EC50 for this effect is 0.049ug/mL.
• Molecular Mass: 44.2kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >80%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 month.
• Reconstitution: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: C1QC complement component 1, q subcomponent, C chain [ Homo sapiens ]
• Official Symbol: C1QC
• Synonyms: C1QC; complement component 1, q subcomponent, C chain; C1QG, complement component 1, q subcomponent, gamma polypeptide; complement C1q subcomponent subunit C; complement component 1, q subcomponent, gamma polypeptide; C1QG; C1Q-C; FLJ27103
• Gene ID: 714
• mRNA Refseq: NM_001114101
• Protein Refseq: NP_001107573
• MIM: 120575
• UniProt ID: P02747