Recombinant Human CA12 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CA12-3407H
• Product Overview: CA12 MS Standard C13 and N15-labeled recombinant protein (NP_001209) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 39.5 kDa
• AA Sequence: MPRRSLHAAAVLLLVILKEQPSSPA PVNGSKWTYFGPDGENSWSKKYPSC GGLLQSPIDLHSDILQYDASLTPLE FQGYNLSANKQFLLTNNGHSVKLNL PSDMHIQGLQSRYSATQLHLHWGNP NDPHGSEHTVSGQHFAAELHIVHYN SDLYPDASTASNKSEGLAVLAVLIE MGSFNPSYDKIFSHLQHVKYKGQEA FVPGFNIEELLPERTAEYYRYRGSL TTPPCNPTVLWTVFRNPVQISQEQL LALETALYCTHMDDPSPREMINNFR QVQKFDERLVYTSFSQVQVCTAAGL SLGIILSLALAGILGICIVVVVSIW LFRRKSIKKGDNKGVIYKPATKMET EAHATRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CA12 carbonic anhydrase XII [ Homo sapiens (human) ]
• Official Symbol: CA12
• Synonyms: CA12; carbonic anhydrase XII; carbonic anhydrase 12; HsT18816; CA-XII; carbonic dehydratase; carbonate dehydratase XII; tumor antigen HOM-RCC-3.1.3; CAXII; FLJ20151
• Gene ID: 771
• mRNA Refseq: NM_001218
• Protein Refseq: NP_001209
• MIM: 603263
• UniProt ID: O43570