Active Recombinant Mouse Carbonic Anhydrase 8, His-tagged

• Cat.No.: Car8-1804M
• Product Overview: A DNA sequence encoding the mouse Car8 (P28651)( Met 1-Gln 291) was expressed, with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: Mouse
• Tag: His
• Protein Length: 1-291 a.a.
• Description: Car8 are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. Car8 participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Purity: > 88 % as determined by SDS-PAGE
• Bio-activity: Measured by its esterase activity. The specific activity is > 5 pmoles/min/μg.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C.
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant mouse Car8 comprises 301 amino acids and has a calculated molecular mass of 34.5 kDa. The recombinant protein migrates as an approximately 37 kDa band in SDS-PAGE under reducing condition.
• Application: SDS-PAGE
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products.
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Pathways: Nitrogen metabolism

Gene Information

• Gene Name: Car8 carbonic anhydrase 8 [ Mus musculus ]
• Official Symbol: Car8
• Synonyms: Car8; carbonic anhydrase 8; Ca8; wdl; Cals; Carp; Cals1; AW546993; carbonic anhydrase-related protein; CA-VIII; CA-RP VIII; OTTMUSP00000004709; carbonic anhydrase VIII; carbonic anhydrase-like sequence 1; NP_031618.2; EC 4.2.1.1
• Gene ID: 12319
• mRNA Refseq: NM_007592
• Protein Refseq: NP_031618
• UniProt ID: P28651
• Chromosome Location: 4
• Function: carbonate dehydratase activity; metal ion binding; protein binding; zinc ion binding