Recombinant Human CABYR Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CABYR-3915H
• Product Overview: CABYR MS Standard C13 and N15-labeled recombinant protein (NP_036321) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: To reach fertilization competence, spermatozoa undergo a series of morphological and molecular maturational processes, termed capacitation, involving protein tyrosine phosphorylation and increased intracellular calcium. The protein encoded by this gene localizes to the principal piece of the sperm flagellum in association with the fibrous sheath and exhibits calcium-binding when phosphorylated during capacitation. A pseudogene on chromosome 3 has been identified for this gene. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 52.6 kDa
• AA Sequence: MISSKPRLVVPYGLKTLLEGISRAV LKTNPSNINQFAAAYFQELTMYRGN TTMDIKDLVKQFHQIKVEKWSEGTT PQKKLECLKEPGKTSVESKVPTQME KSTDTDEDNVTRTEYSDKTTQFPSV YAVPGTEQTEAVGGLSSKPATPKTT TPPSSPPPTAVSPEFAYVPADPAQL AAQMLGKVSSIHSDQSDVLMVDVAT SMPVVIKEVPSSEAAEDVMVAAPLV CSGKVLEVQVVNQTSVHVDLGSQPK ENEAEPSTASSVPLQDEQEPPAYDQ APEVTLQADIEVMSTVHISSVYNDV PVTEGVVYIEQLPEQIVIPFTDQVA CLKENEQSKENEQSPRVSPKSVVEK TTSGMSKKSVESVKLAQLEENAKYS SVYMEAEATALLSDTSLKGQPEVPA QLLDAEGAIKIGSEKSLHLEVEITS IVSDNTGQEESGENSVPQEMEGKPV LSGEAAEAVHSGTSVKSSSGPFPPA PEGLTAPEIEPEGESTAETRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CABYR calcium binding tyrosine phosphorylation regulated [ Homo sapiens (human) ]
• Official Symbol: CABYR
• Synonyms: CABYR; calcium binding tyrosine phosphorylation regulated; CT88; FSP2; CBP86; FSP-2; CABYRa; CABYRc; CABYRe; CABYRc/d; calcium-binding tyrosine phosphorylation-regulated protein; calcium binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2); calcium-binding protein 86; cancer/testis antigen 88; fibrousheathin II; fibrousheathin-2; testis tissue sperm-binding protein Li 84P; testis-specific calcium-binding protein CBP86
• Gene ID: 26256
• mRNA Refseq: NM_012189
• Protein Refseq: NP_036321
• MIM: 612135
• UniProt ID: O75952