Description : |
Calpains constitute a family of intracellular calcium-dependent cysteine proteases. There are eight members in this superfamily. They consist of a variable 80 kDa subunit and an invariant 30 kDa subunit. This calpain protein appears to have protease activity and calcium-binding ability. A similar mouse protein may play a functional role in spermatogenesis and in the regulation of calcium-dependent signal transduction events during meiosis. |
Source : |
E. coli |
Species : |
Human |
Tag : |
His & T7 |
Form : |
Freeze-dried powder |
Molecular Mass : |
24kDa as determined by SDS-PAGE reducing conditions. |
Protein length : |
Phe539~Gly739 (Accession # Q9UMQ6) |
Endotoxin : |
<1.0EU per 1ug (determined by the LAL method) |
Purity : |
>90% |
Applications : |
SDS-PAGE; WB; ELISA; IP. |
Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage buffer : |
Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
Reconstitution : |
Reconstitute in sterile ddH2O. |
Isoelectric Point : |
5.3 |