Recombinant Human CASP10

• Cat.No.: CASP10-26813TH
• Product Overview: Active Recombinant Human Caspase-10/a

Specification

• Description: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Source: E. coli
• Tissue specificity: Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
• Biological activity: SPECIFIC ACTIVITY: 8000 units/mgOne unit of the recombinant caspase-10/a is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C.
• Form: Lyophilised:Reconstitute to 1 unit per μl in PBS containing 15% glycerol.Following reconstitution in PBS and 15% glycerol, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 15% Glycerol, PBS
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the peptidase C14A family.Contains 2 DED (death effector) domains.
• Tag: Non

Gene Information

• Gene Name: CASP10 caspase 10, apoptosis-related cysteine peptidase [ Homo sapiens ]
• Official Symbol: CASP10
• Synonyms: CASP10; caspase 10, apoptosis-related cysteine peptidase; caspase 10, apoptosis related cysteine protease; caspase-10; MCH4
• Gene ID: 843
• mRNA Refseq: NM_001206524
• Protein Refseq: NP_001193453
• MIM: 601762
• Uniprot ID: Q92851
• Chromosome Location: 2q33-q34
• Pathway: Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem; Caspase cascade in apoptosis, organism-specific biosystem
• Function: cysteine-type endopeptidase activity; peptidase activity; protein binding