Recombinant Human Caspase 4, Apoptosis-related Cysteine Peptidase

• Cat.No.: CASP4-599H
• Product Overview: Recombinant Human CASP4 was produced inE. coli.

Specification

• Cat. No.: CASP4-599H
• Description: Caspase-4 is a member of the caspase family of cysteine proteases. It exists in cells as an inactive pro-enzyme. The pro-enzyme is matured by proteolysis to yield large and small subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. The expressed caspase-4 spontaneously undergoes auto-processing to yield subunits characteristic of the native enzyme. The active caspase-4 is routinely tested for its ability to enzymatically cleave the substrate WEHD-AFC or WEHD-pNA.
• Source: E. coli.
• Purity: ≥95% (SDS-PAGE).
• Formulation: Lyophilized.
• Reconstitution: Reconstitute with PBS containing 15% glycerol to 1U/µl.
• Specific Activity: ~5"000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
• Application: Useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
• Storage: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80℃.

Gene Information

• Gene Name: CASP4 caspase 4, apoptosis-related cysteine peptidase [ Homo sapiens ]
• Synonyms: CASP4; caspase 4, apoptosis-related cysteine peptidase; TX; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II; caspase-4; CASP-4; ICE(rel)-II; protease TX; protease ICH-2; apoptotic cysteine protease Mih1/TX; caspase 4, apoptosis-related cysteine protease; EC 3.4.22.57;TX protease; Caspase-4 subunit 1; Caspase-4 subunit 2; ICH2
• Gene ID: 837
• mRNA Refseq: NM_001225
• Protein Refseq: NP_001216
• MIM: 602664
• UniProt ID: P49662
• Chromosome Location: 11q22.2-q22.3
• Function: cysteine-type endopeptidase activity; peptidase activity; protein binding