Recombinant Human CAST Protein (1-667 aa), His-tagged

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Recombinant Human CAST Protein (1-667 aa), His-tagged

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Cat.No. : CAST-1350H
Product Overview : Recombinant Human CAST Protein (1-667 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Neuroscience. Protein Description: Full Length of isoform 4.
Description : Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmort tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Source : Yeast
Species : Human
Tag : His
Form : Tris-based buffer,50% glycerol
Molecular Mass : 73.9 kDa
Protein length : 1-667 aa
AA Sequence : MNPTETKAVK TEPEKKSQST KPKSLPKQAS DTGSNDAHNK KAVSRSAEQQ PSEKSTEPKT KPQDMISAGG ESVAGITAIS GKPGDKKKEK KSLTPAVPVE SKPDKPSGKS GMDAALDDLI DTLGGPEETE EENTTYTGPE VSDPMSSTYI EELGKREVTI PPKYRELLAK KEGITGPPAD SSKPIGPDDA IDALSSDFTC GSPTAAGKKT EKEESTEVLK AQSAGTVRSA APPQEKKRKV EKDTMSDQAL EALSASLGTR QAEPELDLRS IKEVDEAKAK EEKLEKCGED DETIPSEYRL KPATDKDGKP LLPEPEEKPK PRSESELIDE LSEDFDRSEC KEKPSKPTEK TEESKAAAPA PVSEAVCRTS MCSIQSAPPE PATLKGTVPD DAVEALADSL GKKEADPEDG KPVMDKVKEK AKEEDREKLG EKEETIPPDY RLEEVKDKDG KPLLPKESKE QLPPMSEDFL LDALSEDFSG PQNASSLKFE DAKLAAAISE VVSQTPASTT QAGAPPRDTS QSDKDLDDAL DKLSDSLGQR QPDPDENKPM EDKVKEKAKA EHRDKLGERD DTIPPEYRHL LDDNGQDKPV KPPTKKSEDS KKPADDQDPI DALSGDLDSC PSTTETSQNT AKDKCKKAAS SSKAPKNGGK AKDSAKTTEE TSKPKDD
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : CAST calpastatin [ Homo sapiens ]
Official Symbol : CAST
Synonyms : CAST; calpastatin; BS-17; MGC9402;
Gene ID : 831
mRNA Refseq : NM_001042440
Protein Refseq : NP_001035905
MIM : 114090
UniProt ID : P20810

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