Recombinant Human CCNA1 Protein, GST-Tagged

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Recombinant Human CCNA1 Protein, GST-Tagged

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Cat.No. : CCNA1-0647H
Product Overview : Human CCNA1 full-length ORF (AAH36346, 1 a.a. - 464 a.a.) recombinant protein with GST-tag at N-terminal.
Description : The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 76.78 kDa
AA Sequence : METGFPAIMY PGSFIGGWGE EYLSWEGPGL PDFVFQQPVE SEAMHCSNPK SGVVLATVAR GPDACQILTR APLGQDPPQR TVLGLLTANG QYRRTCGQGI TRIRCYSGSE NAFPPAGKKA LPDCGVQEPP KQGFDIYMDE LEQGDRDSCS VREGMAFEDV YEVDTGTLKS DLHFLLDFNT VSPMLVDSSL LSQSEDISSL GTDVINVTEY AEEIYQYLRE AEIRHRPKAH YMKKQPDITE GMRTILVDWL VEVGEEYKLR AETLYLAVNF LDRFLSCMSV LRGKLQLVGT AAMLLASKYE EIYPPEVDEF VYITDDTYTK RQLLKMEHLL LKVLAFDLTV PTTNQFLLQY LRRQGVCVRT ENLAKYVAEL SLLEADPFLK YLPSLIAAAA FCLANYTVNK HFWPETLAAF TGYSLSEIVP CLSELHKAYL DIPHRPQQAI REKYKASKYL CVSLMEPPAV LLLQ
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : CCNA1 cyclin A1 [ Homo sapiens ]
Official Symbol : CCNA1
Synonyms : CCNA1; cyclin A1; cyclin-A1;
Gene ID : 8900
mRNA Refseq : NM_001111045
Protein Refseq : NP_001104515
MIM : 604036
UniProt ID : P78396

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