Recombinant Human CCNE1, Gly & Pro tagged

• Cat.No.: CCNE1-522H
• Product Overview: Recombinant Human CCNE1 (NP_001229.1) (Met1-Ala410), fused with two additional amino acids (Gly & Pro) at the N-terminus, was produced in Baculovirus-Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-410 a.a.
• Predicted N Terminal: Gly
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human CCNE1 consists of 412 amino acids and predicts a molecular mass of 47.2 KDa. The apparent molecular mass of the protein is approximately 48 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CCNE1 cyclin E1 [ Homo sapiens ]
• Official Symbol: CCNE1
• Gene ID: 898
• mRNA Refseq: NM_001238
• Protein Refseq: NP_001229
• MIM: 123837
• UniProt ID: P24864
• Chromosome Location: 19q12