Active Recombinant Human CCNE1 protein, His & GST-tagged

• Cat.No.: CCNE1-259H
• Product Overview: Active Recombinant Human CCNE1 protein(NP_001229.1) (Met 1-Ala 410) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-410 aa
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, 1mM GSH, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human HG3C-CCNE1 at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4, The EC50 of biotinylated human GST-CDK4 is 45.0-106.0 ng/ml.
• Molecular Mass: The recombinant human CCNE1/GST chimera consists of 647 amino acids and has a calculated molecular mass of 75 KDa. It migrates as an apximately 70 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 82 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001229.1
• Unit ID: P24864
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P24864

Gene Information

• Gene Name: CCNE1 cyclin E1 [ Homo sapiens ]
• Official Symbol: CCNE1
• Synonyms: CCNE1; cyclin E1; CCNE; G1/S-specific cyclin-E1; cyclin Es; cyclin Et
• Gene ID: 898
• mRNA Refseq: NM_001238
• protein Refseq: NP_001229
• MIM: 123837