Active Recombinant Human L1CAM protein, His-tagged

• Cat.No.: L1CAM-3144H
• Product Overview: Active Recombinant Human L1CAM protein(P32004-1)(Ile20-Glu1120) is expressed from HEK293 with His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 20-1120 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human L1CAM, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-L1CAM Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 124.6 kDa. Due to glycosylation, the protein migrates to 150-180 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: L1CAM L1 cell adhesion molecule [ Homo sapiens ]
• Official Symbol: L1CAM
• Synonyms: L1CAM; L1 cell adhesion molecule; antigen identified by monoclonal R1, HSAS, HSAS1, MASA, MIC5, S10, SPG1; neural cell adhesion molecule L1; CD171; antigen identified by monoclonal R1; S10; HSAS; MASA; MIC5; SPG1; CAML1; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• Gene ID: 3897
• mRNA Refseq: NM_000425
• Protein Refseq: NP_000416
• MIM: 308840
• UniProt ID: P32004