Active Recombinant Human CD180 protein, His-tagged

• Cat.No.: CD180-3900H
• Product Overview: Active Recombinant Human CD180 protein(NP_005573.2)(Met 1-Gly 626) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-626 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human CD180 at 2 μg/ml (100 μl/well) can bind biotinylated mouse MD-1 with a linear ranger of 3.125-25 μg/ml.
• Molecular Mass: The recombinant human CD180 consists of 614 amino acids and predictes a molecular mass of 69 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD180 is approximately 90-100 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD180 CD180 molecule [ Homo sapiens ]
• Official Symbol: CD180
• Synonyms: CD180; CD180 molecule; CD180 antigen, LY64, lymphocyte antigen 64 (mouse) homolog, radioprotective, 105kD; CD180 antigen; Ly78; RP105; lymphocyte antigen 64; radioprotective 105 kDa protein; lymphocyte antigen-64, radioprotective, 105kDa; LY64; MGC126233; MGC126234
• Gene ID: 4064
• mRNA Refseq: NM_005582
• Protein Refseq: NP_005573
• MIM: 602226
• UniProt ID: Q99467