Recombinant Human CD1D cell lysate
Cat.No. : | CD1D-2479HCL |
Product Overview : | Human CD1D / R3G1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | CD1D CD1d molecule [ Homo sapiens ] |
Official Symbol : | CD1D |
Synonyms : | CD1D; CD1d molecule; CD1d antigen , CD1D antigen, d polypeptide; antigen-presenting glycoprotein CD1d; R3G1; thymocyte antigen CD1D; CD1D antigen, d polypeptide; T-cell surface glycoprotein CD1d; differentiation antigen CD1-alpha-3; HMC class I antigen-like glycoprotein CD1D; R3; CD1A; MGC34622; |
Gene ID : | 912 |
mRNA Refseq : | NM_001766 |
Protein Refseq : | NP_001757 |
MIM : | 188410 |
UniProt ID : | P15813 |
Chromosome Location : | 1q22-q23 |
Pathway : | Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; |
Function : | beta-2-microglobulin binding; cell adhesion molecule binding; eukaryotic cell surface binding; exogenous lipid antigen binding; histone binding; lipid antigen binding; receptor activity; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionSome pharmaceutical companies are researching and developing drugs that target CD1D for therapeutic purposes, although these are still in the early stages of development.
CD1D binds and presents lipid antigens to NKT cells, facilitating immune responses against pathogens or aberrant cells.
CD1D primarily presents lipid antigens, while major histocompatibility complex (MHC) proteins present peptide antigens. CD1D is crucial for lipid-specific immune responses.
CD1D deficiency has been associated with an increased susceptibility to certain infections and a potential link to autoimmune disorders.
Yes, several clinical trials are exploring the modulation of CD1D in various diseases, including cancer and autoimmune disorders, to assess its therapeutic potential.
Customer Reviews (3)
Write a reviewThe CD1D protein's versatility allows it to be effectively employed in various experimental designs, further enhancing its suitability for diverse research needs.
Their extensive knowledge and expertise can help troubleshoot issues or provide guidance on experimental design, enabling you to overcome obstacles and achieve your research goals.
In protein electron microscopy structure analysis, the CD1D protein demonstrates outstanding performance.
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