Active Recombinant Human CD200 protein, hFc-tagged, Low Endotoxin

• Cat.No.: CD200-165H
• Product Overview: Recombinant Human CD200 protein(P41217-1)(Gln31-Gly232), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 31-232 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD200 R1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 49.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD200 CD200 molecule [ Homo sapiens ]
• Official Symbol: CD200
• Synonyms: CD200; CD200 molecule; antigen identified by monoclonal MRC OX 2, CD200 antigen, MOX1, MOX2; OX-2 membrane glycoprotein; MRC; OX 2; CD200 antigen; MRC OX-2 antigen; antigen identified by monoclonal MRC OX-2; MOX1; MOX2; OX-2
• Gene ID: 4345
• mRNA Refseq: NM_001004196
• Protein Refseq: NP_001004196
• MIM: 155970
• UniProt ID: P41217