Active Recombinant Human CD274 Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: CD274-27H
• Product Overview: Biotinylated Active Recombinant Human CD274 Protein(Q9NZQ7) is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 19-239 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Bio-activity: Immobilized Recombinant Human PD-1 (C-mFc) at 2 μg/ml (100 μl/well) can bind Biotinylated Human PD-L1 (C-Fc-Avi). The ED50 of Human Biotinylated Human PD-L1 (C-Fc-Avi) is 4.32 ng/ml.
• Molecular Mass: 54.2 KDa
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Gene Information

• Gene Name: CD274 CD274 molecule [ Homo sapiens ]
• Official Symbol: CD274
• Synonyms: CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296
• Gene ID: 29126
• mRNA Refseq: NM_014143
• Protein Refseq: NP_054862
• MIM: 605402
• UniProt ID: Q9NZQ7