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Active Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated

Cat.No. : CD274-365H
Product Overview : Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1). Predicted N-terminus: Phe 19. This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag is inserted in-between the Fc and his tags. The protein has a calculated MW of 53.2 kDa. The protein migrates as 66-75 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
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Source : HEK293
Species : Human
Tag : His & Fc & Avi
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human PD-1, Fc Tag at 2 μg/mL (100μL/well) can bind Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated with a linear range of 0.01-0.25 μg/mL (QC tested).
Immobilized Human B7-1, mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated with a linear range of 0.02-0.625 μg/mL (Routinely tested).
Flow Cytometry assay shows that recombinant Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 1 μg/ml (Routinely tested).
FACS analysis shows that the binding of Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-PD-1 antibody. The concentration of PD-L1 used is 2μg/ml. The IC50 is 0.64 μg/ml (Routinely tested).
Immobilized cell surface PD-1(5x104 of cells per well) can bind Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated with an EC50 of 0.082ug/ml (Routinely tested).
Molecular Mass : 53.2 kDa
Protein length : Phe 19 - Arg 238
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name : CD274 CD274 molecule [ Homo sapiens ]
Official Symbol : CD274
Synonyms : CD274; CD274 molecule; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296;
Gene ID : 29126
mRNA Refseq : NM_014143
Protein Refseq : NP_054862
MIM : 605402
UniProt ID : Q9NZQ7

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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