Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated

Cat.No.: CD274-365H
Product Overview: Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1). Predicted N-terminus: Phe 19. This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag is inserted in-between the Fc and his tags. The protein has a calculated MW of 53.2 kDa. The protein migrates as 66-75 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Source: HEK293
Species: Human
Tag : His & Fc & Avi
Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity: Immobilized Human PD-1, Fc Tag at 2 μg/mL (100μL/well) can bind Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated with a linear range of 0.01-0.25 μg/mL (QC tested).
Immobilized Human B7-1, mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated with a linear range of 0.02-0.625 μg/mL (Routinely tested).
Flow Cytometry assay shows that recombinant Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 1 μg/ml (Routinely tested).
FACS analysis shows that the binding of Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-PD-1 antibody. The concentration of PD-L1 used is 2μg/ml. The IC50 is 0.64 μg/ml (Routinely tested).
Immobilized cell surface PD-1(5x104 of cells per well) can bind Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated with an EC50 of 0.082ug/ml (Routinely tested).
Molecular Mass: 53.2 kDa
Protein length: Phe 19 - Arg 238
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Notes: Please avoid repeated freeze-thaw cycles.
Stability: No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name: CD274 CD274 molecule [ Homo sapiens ]
Official Symbol: CD274
Synonyms: CD274; CD274 molecule; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296;
Gene ID: 29126
mRNA Refseq: NM_014143
Protein Refseq: NP_054862
MIM: 605402
UniProt ID: Q9NZQ7

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