Active Recombinant Human CD274 protein, His-tagged
Cat.No. : | CD274-1176H |
Product Overview : | Recombinant Human CD274 protein(Phe19~Arg238), fused with N-terminal His tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose. |
Bio-activity : | Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Besides, Programmed Cell Death Protein 1 (PDCD1) has been identified as an interactor of PDCD1LG1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PDCD1LG1 and recombinant human PDCD1. Briefly, PDCD1LG1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PDCD1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PDCD1LG1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of PDCD1LG1 and PDCD1 was shown in Figure 1, and this effect was in a dose dependent manner. |
Molecular Mass : | 38kDa as determined by SDS-PAGE reducing conditions. |
Protein length : | Phe19~Arg238 |
Purity : | > 95% |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. |
Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name : | CD274 CD274 molecule [ Homo sapiens ] |
Official Symbol : | CD274 |
Synonyms : | CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296; |
Gene ID : | 29126 |
mRNA Refseq : | NM_014143 |
Protein Refseq : | NP_054862 |
MIM : | 605402 |
UniProt ID : | Q9NZQ7 |
Products Types
◆ Recombinant Protein | ||
CD274-705H | Recombinant Human CD274 Protein, C13 and N15 Labeled | +Inquiry |
CD274-0765H | Recombinant Human CD274 Protein, GST-Tagged | +Inquiry |
CD274-1081H | Recombinant Human CD274 protein(Met1-Thr239), His-tagged, Biotinylated | +Inquiry |
CD274-707C | Recombinant Cynomolgus monkey CD274 Protein, Fc-tagged | +Inquiry |
CD274-051H | Active Recombinant Human CD274 protein(Met1-Thr239), His&Avi-tagged, Biotinylated | +Inquiry |
◆ Lysates | ||
CD274-2642MCL | Recombinant Mouse CD274 cell lysate | +Inquiry |
CD274-002RCL | Recombinant Rat CD274 cell lysate | +Inquiry |
CD274-914CCL | Recombinant Cynomolgus CD274 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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