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Recombinant Human CD276 Protein, Fc-Avi-tagged, Biotinylated

Cat.No. : CD276-1287H
Product Overview : Biotinylated Human B7-H3, Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Leu 29 - Pro 245 (Accession # Q5ZPR3-2). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.
Source : HEK293
Species : Human
Tag : Fc-Avi
Predicted N Terminal : Leu 29
Form : Lyophilized from 0.22 um filtered solution in 49 mM Tris, 100 mM Glycine, 25 mM Arg, 150 mM NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 51.6 kDa. The protein migrates as 63-70 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : CD276 CD276 molecule [ Homo sapiens ]
Official Symbol : CD276
Synonyms : CD276; CD276 molecule; CD276 antigen; B7 H3; B7H3; B7RP 2; B7 homolog 3; costimulatory molecule; B7-H3; B7RP-2; 4Ig-B7-H3;
Gene ID : 80381
mRNA Refseq : NM_001024736
Protein Refseq : NP_001019907
MIM : 605715
UniProt ID : Q5ZPR3

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