Cat.No. : |
CD28-32H
Optional Service: Optional requirements on this protein
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Product Overview : |
Human CD28-muIg fusion protein was Protein A purified from (low FBS containing) tissue culture supernatant of CHO transfectants, and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate by diafiltration. A soluble fusion protein consisting of the extracellular (134aa) domain of human CD28 fused to murine IgG2a Fc (233aa). |
Description : |
Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+T cells. T cell activities attributed to CD28 include, prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNAs and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28, identified as CD80 (B7-1) and CD86 (B7-2), are immunoglobulin superfamily monomeric transmembrane glycoproteins of 60 kd and 80 kd, respectively. |
Source : |
CHO |
Species : |
Human |
Bio-activity : |
CD28-muIg/Biotin activity was assessed in EIA utilizing GAM-captured Recombinant CD80-muIg, and Streptavidin/HRP as a detector. In this format, the fusion protein was detectable at 100 ng/ml. |
Notes : |
Research use only. Not for use in Diagnostic procedures. |
Stability : |
Product should retain activity for at least 12 months after shipping date when stored as recommended. |
Storage : |
Store at 2 - 5 centigrade. Freeze/Thawing is not recommended. |
Concentration : |
0.5 mg/mL |
Storage Buffer : |
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative). |