Active Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein, His-Avi-tagged, Low Endotoxin

• Cat.No.: CD28-747H
• Product Overview: Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein(P10747-1)(19-152 aa), fused with N-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human/Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: Avi&His
• Protein Length: 19-152 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 23.9 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD28 CD28 molecule [ Homo sapiens ]
• Official Symbol: CD28
• Synonyms: CD28; CD28 molecule; CD28 antigen (Tp44); T-cell-specific surface glycoprotein CD28; T cell specific surface glycoprotein; CD28 antigen; Tp44; MGC138290
• Gene ID: 940
• mRNA Refseq: NM_001243077
• Protein Refseq: NP_001230006
• MIM: 186760
• UniProt ID: P10747