Recombinant Human CD33 Protein, Fc-tagged, Alexa Fluor 647 conjugated

• Cat.No.: CD33-313HAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant human CD33 (AAH28152.1) (Met1-His259), fused with the Fc region of mouse IgG at the C-terminus, was produced in Human Cells.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Molecular Mass: The recombinant human CD33/mFc is a disulfide-linked homodimer. The reduced monomer comprises 476 amino acids and has a predicted molecular mass of 53.1 kDa. The apparent molecular mass of the protein is approximately 64-68 in SDS-PAGE under reducing cond
• Protein length: 476
• N-terminal Sequence Analysis: Asp 18
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD33 CD33 molecule [ Homo sapiens ]
• Official Symbol: CD33
• Gene ID: 945
• mRNA Refseq: NM_001082618
• Protein Refseq: NP_001076087
• MIM: 159590
• UniProt ID: P20138

Reviews

11/14/2019

Quick dissolution, easy use.

08/27/2019

Excellent solubility, no aggregates.

02/27/2019

High concentration, very effective.

Q&As

What are the implications of CD33 polymorphisms in therapeutic targeting?

Certain polymorphisms in the CD33 gene can influence its expression on the cell surface, which might affect the efficacy of CD33-targeted therapies.

What is the primary biological function of CD33 in hematopoietic cells?

CD33 is a transmembrane receptor primarily expressed on cells of myeloid lineage and is involved in modulating cellular functions, including the regulation of cellular proliferation and differentiation.

How does CD33 expression vary among different leukocyte populations?

CD33 is predominantly expressed on myeloid cells, especially on immature myeloid cells. Its expression decreases as these cells differentiate and is typically low or absent on lymphoid cells.

How has CD33 been utilized as a therapeutic target in hematological malignancies?

CD33 has been targeted for therapeutic antibodies in AML. An example is gemtuzumab ozogamicin, which targets CD33 and delivers a cytotoxic agent specifically to leukemia cells expressing CD33.

How does CD33 signaling influence myeloid cell differentiation?

While the exact signaling pathways of CD33 are still under investigation, it is believed that CD33 signaling can influence the differentiation and function of myeloid cells, potentially playing a role in immune regulation.

What role does CD33 play in acute myeloid leukemia (AML)?

CD33 is commonly expressed on the surface of AML blasts, making it a potential target for therapeutic interventions in AML.

Are there known ligands that bind specifically to CD33?

The specific ligands for CD33 are not fully characterized, but it is believed to play a role in cell-cell interactions within the immune system.