Recombinant Human CD3D & CD3E Heterotrimer Protein (Met1-Ala105 & Met1-Asp126), His-Flag-tagged, Biotinylated
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|Product Overview :||Recombinant Human CD3D & CD3E Heterotrimer Protein (P04234)(Met1-Ala105) & (P07766)(Met1-Asp126) was produced by HEK293 Cells expression system. The CD3D protein was fused with a flag tag at the C-terminus, constructed the plasmid 1. The CD3E protein was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. The purified protein was biotinylated in vitro.|
|Predicted N Terminal :||Phe 22 & Asp 23|
|Form :||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.|
|Molecular Mass :||The recombinant human CD3D&CD3E consists of 272 (126+146) amino acids and predicts a molecular mass of 31.2 (14.4+16.8)KDa.|
|Protein length :||Met1-Ala105 & Met1-Asp126|
|Endotoxin :||< 1.0 EU per μg protein as determined by the LAL method.|
|Purity :||> 95 % as determined by SDS-PAGE.|
|Stability :||Samples are stable for up to twelve months from date of receipt at -70 centigrade.|
|Storage :||Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|Shipping :||In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|Gene Name :||CD3D CD3d molecule, delta (CD3-TCR complex) [ Homo sapiens ] & CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]|
|Official Symbol :||CD3D & CD3E|
|Synonyms :||CD3D; CD3 delta; OKT3, delta chain; T3D; CD3-DELTA & CD3E; T3E; TCRE; FLJ18683;|
|Gene ID :||915 & 916|
|mRNA Refseq :||NM_000732 & NM_000733|
|Protein Refseq :||NP_000723 & NP_000724|
|UniProt ID :||P04234 & P07766|
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