| Description : |
T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His-Avi |
| Predicted N Terminal : |
Asp 23(CD3E) & Phe 22(CD3D) |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
| Bio-activity : |
Immobilized OKT3 at 10 μg/mL (100 μL/well) can bind Biotinylated Human CD3E & CD3D with a linear range of 0.156-5 ug/mL. |
| Molecular Mass : |
Biotinylated Human CD3 epsilon & CD3 delta Heterodimer Protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 18.7 kDa (CD3E), 13.5 kDa (CD3D). The reducing heterodimer protein migrates as 22 kDa (CD3E) and 26 kDa (CD3D) due to glycosylation. |
| Protein length : |
Asp 23-Asp 126(CD3E) & Phe 22-Ala 105(CD3D) |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μl. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
