"CD3E" Related Products

Recombinant Human CD3E & CD3D Protein, His-Avi-tagged, Biotinylated

Cat.No.: CD3E & CD3D-377H
Product Overview: Biotinylated Human CD3 epsilon & CD3 delta (CDD-H82W1) is expressed from HEK293 cells. It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)). Subunit CD3E is fused with a polyhistidine tag and a Avi tag at the C-terminus and subunit CD3D contains no tag.
Source: HEK293
Species: Human
Tag : His-Avi
Predicted N Terminal: Asp 23 (CD3E) & Phe 22 (CD3D)
Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity: Immobilized OKT3 at 10 ug/mL (100 uL/well) can bind Biotinylated Human CD3E & CD3D with a linear range of 0.156-5 ug/mL.
Molecular Mass: Biotinylated Human CD3 epsilon & CD3 delta Heterodimer Protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 18.7 kDa (CD3E), 13.5 kDa (CD3D). The reducing heterodimer protein migrates as 22 kDa (CD3E) and 26 kDa (CD3D) due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90% as determined by SDS-PAGE.
Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Official Symbol: CD3E; CD3D
Synonyms: CD3E & CD3D, CD3 delta & CD3 epsilon, CD3 delta / epsilon

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