Active Recombinant Human CD3E & CD3G protein, His & Fc & Flag-tagged
Cat.No. : | CD3E & CD3G-342H |
Product Overview : | Human CD3 epsilon & CD3 gamma is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1 (CD3E) & AAI13831 (CD3G)). |
- Specification
- Gene Information
- Related Products
Description : | T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. |
Source : | HEK293 |
Species : | Human |
Tag : | His & Fc & Flag |
Predicted N Terminal : | Asp 23 (CD3E) & Gln 23 (CD3G) |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
Bio-activity : | Immobilized Anti-CD3E & CD3G MAb, Mouse IgG2a at 10μg/mL (100 μL/well) can bind Human CD3E & CD3G with a linear range of 0.3-5 μg/mL. |
Molecular Mass : | The heterodimer Human CD3 epsilon & CD3 gamma is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation. |
Protein length : | Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Storage buffer : | PBS, pH7.4 |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Shipping : | Powder,RT |
Gene Name : | CD3D CD3d molecule [ Homo sapiens (human) ] |
Official Symbol : | CD3E; CD3G |
Synonyms : | T3D; IMD19; CD3-DELTA; CD3 antigen, delta subunit; CD3 delta; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); OKT3, delta chain; T-cell receptor T3 delta chain; T3G; IMD17; CD3-GAMMA; CD3G; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 gamma chain |
Gene ID : | 915 |
mRNA Refseq : | NM_000732.4 |
Protein Refseq : | NP_000723.1 |
UniProt ID : | P04234 |
Products Types
◆ Recombinant Protein | ||
CD3E-3081H | Recombinant Human CD3E Protein, MYC/DDK-tagged | +Inquiry |
CD3E-140C | Recombinant Cynomolgus monkey CD3E Protein, His and Fc-tagged | +Inquiry |
Cd3e-322R | Recombinant Rhesus CD3E Protein, His-tagged | +Inquiry |
CD3E-675H | Recombinant Human CD3E Protein, His-tagged | +Inquiry |
CD3E-321H | Recombinant Human CD3E Protein, His-tagged | +Inquiry |
◆ Lysates | ||
CD3E-1275HCL | Recombinant Human CD3E cell lysate | +Inquiry |
CD3E-1602CCL | Recombinant Cynomolgus CD3E cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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