Recombinant Human CD3E Protein, His-Avi-tagged, Biotinylated

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Recombinant Human CD3E Protein, His-Avi-tagged, Biotinylated

CD3E Related Products

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Cat.No. : CD3E-2193H
Product Overview : Biotinylated Human CD3 epsilon, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Asp 23 - Asp 126 (Accession # P07766-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
Source : HEK293
Species : Human
Tag : His-Avi
Predicted N Terminal : Asp 23
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized Anti Human CD3e MAb at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD3 epsilon, His Tag, Avi Tag with a linear range of 0.2-6 ug/mL.
Molecular Mass : The protein has a calculated MW of 15.5 kDa. The protein migrates as 17-18 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
Official Symbol : CD3E
Synonyms : CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683;
Gene ID : 916
mRNA Refseq : NM_000733
Protein Refseq : NP_000724
UniProt ID : P07766
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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