| Cat.No. : |
CD4-313H |
| Product Overview : |
Recombinant Human CD4 Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # P01730-1). Predicted N-terminus: Lys 26. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His & Avi |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized HIV GP120 (HIV), His Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human CD4 protein, His & Avi-tagged, Biotinylated with a linear range of 2-39 ng/mL (QC tested).
Immobilized HIV GP120 (HIV), His Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human CD4 protein, His & Avi-tagged, Biotinylated with a linear range of 2-78 ng/mL (Routinely tested). |
| Molecular Mass : |
45.0 kDa |
| Protein length : |
Lys 26 - Pro 396 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Notes : |
Please avoid repeated freeze-thaw cycles. |
| Stability : |
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
| Figure : |
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