Recombinant Human CD47 protein, His-tagged, low endotoxin

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Recombinant Human CD47 protein, His-tagged, low endotoxin

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Cat.No. : CD47-3292H
Product Overview : Recombinant Human CD47 (Gln19-Pro139) protein (low endotoxin) was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Gln19
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 15.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gln19-Pro139
Endotoxin : Less than 0.01 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CD47-3292H :

Human CD47, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of CD47-3292H :

Immobilized Human SIRP alpha, Fc Tag at 5 μg/mL (100 μL/well) can bind Human CD47, His Tag, low endotoxin with a linear range of 2-13 ng/mL (QC tested).

Bioactivity-ELISA :

Immobilized Anti-CD47 MAb, Human IgG4 (clone 5F9) at 2 μg/mL (100 μL/well) can bind Human CD47, His Tag, low endotoxin with a linear range of 0.4-3 ng/mL (Routinely tested).

Gene Name : CD47
Official Symbol : CD47
Synonyms : CD47; CD47 molecule; CD47 antigen (Rh related antigen, integrin associated signal transducer) , MER6; leukocyte surface antigen CD47; antigen identified by monoclonal 1D8; antigenic surface determinant protein OA3; CD47 glycoprotein; IAP; integrin associated protein; OA3; Rh related antigen; Rh-related antigen; integrin-associated protein; integrin-associated signal transducer; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); MER6
Gene ID : 961
mRNA Refseq : NM_001777
Protein Refseq : NP_001768
MIM : 601028
UniProt ID : Q08722

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