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Recombinant Human CD70 Protein, Fc-Avi-tagged, Biotinylated

Cat.No.: CD70-892H
Product Overview: Biotinylated Human CD27 Ligand, Fc Tag is expressed from HEK293 cells. It contains AA Gln 39 - Pro 193 (Accession # P32970-1). This protein carries an Avi tag at the N-terminus, followed by a human IgG1 Fc tag.
Source: HEK293
Species: Human
Tag : Fc-Avi
Predicted N Terminal: Gly
Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Bio-activity: Immobilized Human CD27, Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag with a linear range of 1-31 ug/mL.
Molecular Mass: The protein has a calculated MW of 45 kDa. The protein migrates as 50-60 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >85% as determined by SDS-PAGE.
Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: CD70 CD70 molecule [ Homo sapiens ]
Official Symbol: CD70
Synonyms: CD70; CD70 molecule; CD27LG, TNFSF7, tumor necrosis factor (ligand) superfamily, member 7; CD70 antigen; CD27L; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7; CD27LG; TNFSF7;
Gene ID: 970
mRNA Refseq: NM_001252
Protein Refseq: NP_001243
MIM: 602840
UniProt ID: P32970

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